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Browsing by Author Vo, Thi Thuong Lan

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  • Aberrant Promoter Methylation of BRCA1 and RASSF1A in Tumor and Paired Adjacent Normal Tissues from Vietnamese Patients with Breast Cancer

    • Authors: Ngo, Thi Ha; Doan, Thi Hong Van; Le, Thi Thu Ha; Ta, Bich Thuan; Vo, Thi Thuong Lan (2016)

  • DNA promoter methylation, a main way of epigenetic regulation, has been studied for detection, prognosis and treatment of breast cancer. In this study, methylation specific polymerase chain reaction (MSP) was used to analyze the promoter methylation of 2 tumor suppressor genes BRCA1 and RASSF1A in tumor and paired adjacent normal tissues of 76 Vietnamese breast cancer patients. We found that tumor and paired adjacent normal tissues were frequently hypermethylated for the two tested genes. The BRCA1 and RASSF1A were highly methylated in tumors (60.5% and 76.3%) and adjacent normal tissues (52.6% and 65.8%), respectively. Further more, there was a high agreement between BRCA1 and RASSF1...
  • document(54).pdf.jpg
  • Article

  • Detection of Common Beta-thalassemia Mutations by Reverse Dot Blot Analysis

    • Authors: Nguyen, Thu Trang; Le, Thi Thu Ha; Nguyen, Thuy Ngan; Trieu, Tien Sang; Pham, Anh Thuy Duong; Vo, Thi Thuong Lan (2016)

  • Beta (β)-thalassemia is the most common genetic disease of anemia caused by mutations on beta globin gene. In Vietnam, there is a high frequency of β -thalassemia carriers with a prevalence ranging from 1.5 to 25.0 % in the different ethnic groups. The most common mutations are the nonsense in codon (CD) 17 (A>T), codon 26 (G>A) and the frameshift at codons 41/42 (-TTCT). The polymerase chain reaction-amplification refractory mutation system (PCR-ARMS) is challenged by using a great number of primer sets for detecting normal and mutated alleles. Reverse dot-blot hybridization using oligonucleotide probes can simultaneously detect allelic specific mutations on a membrane. In this study...
  • #4-DDL_2015_QT.pdf.jpg
  • Article

  • Interaction of Vietnamese Medicinal Plant Extracts with Recombinantly Expressed Human Neurokinin-1 Receptor

    • Authors: Dinh, Doan Long; Pham, Thi Hong Nhung; Hoang, Thi My Nhung; Trinh, Tat Cuong; Nguyen, Thi Hong Van; Vo, Thi Thuong Lan; Pham, Thanh Huyen; Lundstrom, Kenneth (2015)

  • A primary functional receptor screening assay was performed to examine the effects of methanolic extracts from ten Vietnamese medicinal plants on the human neurokinin-1 receptor expressed from Semliki Forest virus vectors in Chinese hamster ovary cells. Extracts from Piper nigrum, Stephania cambodica and Styphnolobium japonicum were found to exert inhibition on agonist-induced human neurokinin-1 receptor activity. Secondary assays and high performance liquid chromatography of the lead compounds addressed a possible association between pharmacological responses and these chemical compounds. Strong inhibition of human neurokinin-1 receptor was observed for extracts revealing the highest...
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  • Article

  • Methylation of miRNA-34a Promoter: a Preliminary Study in Vietnamese Breast Cancer Patients

    • Authors: Pham, Anh Thuy Duong; Thieu, Minh Thu; Vo, Thi Thuong Lan (2016)

  • Methylation of miRNA-34a (miR-34a) promoter, a target gene of p53 which induces apoptosis, cell cycle arrest or senescence, was reported in many cancer types, including breast cancer, one of the most common malignancies in women. However there has not been any study on miR-34a methylation in Vietnam. Therefore, this research provides the first sight of methylation status of miR-34a promoter in breast cancer in Vietnam. By using methyl-specific PCR (MSP) technique, we found that the frequency of miR-34a promoter methylation in breast tumors and adjacent tissues was 43.3% (13/30) and 13.3% (4/30), respectively. At the transcriptional level, two target genes of miR-34a, AXL and MDM4, wer...
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  • Article

  • Screening Streptomycin Resistant Mutations from Gamma Ray Irradiated Bacillus subtilis B5 for Selection of Potential Mutants with high Production of Protease

    • Authors: Tran, Bang Diep; Nguyen, Thi Thom; Hoang, Dang Sang; Hoang, Phuong Thao; Nguyen, Van Binh; Ta, Bich Thuan; Vo, Thi Thuong Lan; Tran, Minh Quynh (2016)

  • The suspensions of Bacillus subtilis B5, a rather high protease production strain, in logarithmic growth phase were irradiated under gamma Cobalt-60 source at Hanoi Irradiation Center. After treatment, the irradiated cells were intermediately cultured in the nutrient agar plates supplemented without and with 20µg/ml streptomycin for screening. The radiation effects on their viability and mutant frequency were studied with radiation dose. The results showed that its survival rate was reduced with the dose as biphasic function. The cells irradiated at dose higher than 1200 Gy do not form colony in the medium containing streptomycin though they could survive in nutrient agar. Therefore...

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Browsing by Author Vo, Thi Thuong Lan

Jump to: 0-9 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z
or enter first few letters:  
Showing results 1 to 5 of 5
  • document(26).pdf.jpg
  • Article

  • Aberrant Promoter Methylation of BRCA1 and RASSF1A in Tumor and Paired Adjacent Normal Tissues from Vietnamese Patients with Breast Cancer

    • Authors: Ngo, Thi Ha; Doan, Thi Hong Van; Le, Thi Thu Ha; Ta, Bich Thuan; Vo, Thi Thuong Lan (2016)

  • DNA promoter methylation, a main way of epigenetic regulation, has been studied for detection, prognosis and treatment of breast cancer. In this study, methylation specific polymerase chain reaction (MSP) was used to analyze the promoter methylation of 2 tumor suppressor genes BRCA1 and RASSF1A in tumor and paired adjacent normal tissues of 76 Vietnamese breast cancer patients. We found that tumor and paired adjacent normal tissues were frequently hypermethylated for the two tested genes. The BRCA1 and RASSF1A were highly methylated in tumors (60.5% and 76.3%) and adjacent normal tissues (52.6% and 65.8%), respectively. Further more, there was a high agreement between BRCA1 and RASSF1...
  • document(54).pdf.jpg
  • Article

  • Detection of Common Beta-thalassemia Mutations by Reverse Dot Blot Analysis

    • Authors: Nguyen, Thu Trang; Le, Thi Thu Ha; Nguyen, Thuy Ngan; Trieu, Tien Sang; Pham, Anh Thuy Duong; Vo, Thi Thuong Lan (2016)

  • Beta (β)-thalassemia is the most common genetic disease of anemia caused by mutations on beta globin gene. In Vietnam, there is a high frequency of β -thalassemia carriers with a prevalence ranging from 1.5 to 25.0 % in the different ethnic groups. The most common mutations are the nonsense in codon (CD) 17 (A>T), codon 26 (G>A) and the frameshift at codons 41/42 (-TTCT). The polymerase chain reaction-amplification refractory mutation system (PCR-ARMS) is challenged by using a great number of primer sets for detecting normal and mutated alleles. Reverse dot-blot hybridization using oligonucleotide probes can simultaneously detect allelic specific mutations on a membrane. In this study...
  • #4-DDL_2015_QT.pdf.jpg
  • Article

  • Interaction of Vietnamese Medicinal Plant Extracts with Recombinantly Expressed Human Neurokinin-1 Receptor

    • Authors: Dinh, Doan Long; Pham, Thi Hong Nhung; Hoang, Thi My Nhung; Trinh, Tat Cuong; Nguyen, Thi Hong Van; Vo, Thi Thuong Lan; Pham, Thanh Huyen; Lundstrom, Kenneth (2015)

  • A primary functional receptor screening assay was performed to examine the effects of methanolic extracts from ten Vietnamese medicinal plants on the human neurokinin-1 receptor expressed from Semliki Forest virus vectors in Chinese hamster ovary cells. Extracts from Piper nigrum, Stephania cambodica and Styphnolobium japonicum were found to exert inhibition on agonist-induced human neurokinin-1 receptor activity. Secondary assays and high performance liquid chromatography of the lead compounds addressed a possible association between pharmacological responses and these chemical compounds. Strong inhibition of human neurokinin-1 receptor was observed for extracts revealing the highest...
  • document(20).pdf.jpg
  • Article

  • Methylation of miRNA-34a Promoter: a Preliminary Study in Vietnamese Breast Cancer Patients

    • Authors: Pham, Anh Thuy Duong; Thieu, Minh Thu; Vo, Thi Thuong Lan (2016)

  • Methylation of miRNA-34a (miR-34a) promoter, a target gene of p53 which induces apoptosis, cell cycle arrest or senescence, was reported in many cancer types, including breast cancer, one of the most common malignancies in women. However there has not been any study on miR-34a methylation in Vietnam. Therefore, this research provides the first sight of methylation status of miR-34a promoter in breast cancer in Vietnam. By using methyl-specific PCR (MSP) technique, we found that the frequency of miR-34a promoter methylation in breast tumors and adjacent tissues was 43.3% (13/30) and 13.3% (4/30), respectively. At the transcriptional level, two target genes of miR-34a, AXL and MDM4, wer...
  • document(19).pdf.jpg
  • Article

  • Screening Streptomycin Resistant Mutations from Gamma Ray Irradiated Bacillus subtilis B5 for Selection of Potential Mutants with high Production of Protease

    • Authors: Tran, Bang Diep; Nguyen, Thi Thom; Hoang, Dang Sang; Hoang, Phuong Thao; Nguyen, Van Binh; Ta, Bich Thuan; Vo, Thi Thuong Lan; Tran, Minh Quynh (2016)

  • The suspensions of Bacillus subtilis B5, a rather high protease production strain, in logarithmic growth phase were irradiated under gamma Cobalt-60 source at Hanoi Irradiation Center. After treatment, the irradiated cells were intermediately cultured in the nutrient agar plates supplemented without and with 20µg/ml streptomycin for screening. The radiation effects on their viability and mutant frequency were studied with radiation dose. The results showed that its survival rate was reduced with the dose as biphasic function. The cells irradiated at dose higher than 1200 Gy do not form colony in the medium containing streptomycin though they could survive in nutrient agar. Therefore...