Browsing by Subject lipase,

Jump to: 0-9 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z
or enter first few letters:  
Showing results 1 to 1 of 1
  • document(40).pdf.jpg
  • Article


  • Authors: Nguyen, Thi Huyen; Trinh, Le Phuong; Do, Thi Thanh Trung; Le, Hong Diep; Pham, Bao Yen (2016)

  • Successful expression of target genes, often indicated by high yield and solubility, is critical for studies involving recombinant proteins. Yet the most common bacterial expression system utilizing Escherichia coli as host cells is usually reported to produce low amounts of soluble target proteins. In this study, two Helicobacter pylori (Hp) genes, Hp lipase and Hp peptide deformylase (Hp-PDF), whose encoded proteins are crucial for bacterial growth and colonization, thus could be used to screen potential anti-Hp drugs, were designed to be expressed in such system. Genetic engineering, experimental biology, and computational biology methods were employed to enhance recombinant protei...

Browsing by Subject lipase,

Jump to: 0-9 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z
or enter first few letters:  
Showing results 1 to 1 of 1
  • document(40).pdf.jpg
  • Article


  • Authors: Nguyen, Thi Huyen; Trinh, Le Phuong; Do, Thi Thanh Trung; Le, Hong Diep; Pham, Bao Yen (2016)

  • Successful expression of target genes, often indicated by high yield and solubility, is critical for studies involving recombinant proteins. Yet the most common bacterial expression system utilizing Escherichia coli as host cells is usually reported to produce low amounts of soluble target proteins. In this study, two Helicobacter pylori (Hp) genes, Hp lipase and Hp peptide deformylase (Hp-PDF), whose encoded proteins are crucial for bacterial growth and colonization, thus could be used to screen potential anti-Hp drugs, were designed to be expressed in such system. Genetic engineering, experimental biology, and computational biology methods were employed to enhance recombinant protei...