Please use this identifier to cite or link to this item: http://repository.vnu.edu.vn/handle/VNU_123/26637
Title: Synthesis and surface functionalization of Fe3O4-SiO2 core-shell nanoparticles with 3-glycidoxypropyltrimethoxysilane and 1,1 '-carbonyldiimidazole for bio-applications
Authors: Ta, Thi Kieu Hanh
Trinh, Minh-Thuong
Nguyen, Viet Long
Nguyen, Thi Thanh My
Nguyen, Thi Lien Thuong
Keywords: Core-shell Fe3O4-SiO2 nanoparticles;Fe3O4-SiO2-GPS-CDI-pA/G-T;3-Glycidoxypropyltrimethoxysilane;Protein BSA
Issue Date: 2016
Publisher: H. : ĐHQGHN
Citation: ISIKNOWLEDGE
Abstract: In our research, we have presented the controlled synthesis of Fe3O4 nanoparticles (NPs) with a size of about 10 nm coated with SiO2 shells for bio-applications. On this basis, the controlled synthesis of Fe3O4-SiO2 core-shell nanoparticles and their surface functionalization with 3-glycidoxypropyltrimethoxysilane (GPS) and 1,1 ’ -carbonyldiimidazole (CDI) has been presented with a facile synthetic process. The as-prepared Fe3O4-SiO2-GPS-CDI core-multishell NPs can bind proteins. Therefore, recombinant protein A/G (pA/G) was efficiently coupled onto the surface of NPs via CDI groups, creating a complete coverage. Antibodies (T IgG) were also conjugated on Fe3O4-SiO2-GPS-CDI-pA/G, i.e. 9 mg T antibodies per 1g NPs. After the surface functionalization of the magnetic nanoparticles, their superparamagnetism was reduced by a factor of about threefold in Fe3O4-SiO2, and fivefold in Fe3O4- SiO2-GPS-CDI-pA/G in comparison with that of the naked Fe3O4 NPs. The NPs conjugated with T IgG could bind and remove 1x10 5 cells 3 per 0.25 mg NPs in vitro. Finally, the new models of surface functionalization of magnetic nanoparticles have been proposed for promising bioconjugations in our further research.
Description: COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS Volume: 504 Pages: 376-383 ; TNS06369
URI: http://repository.vnu.edu.vn/handle/VNU_123/26637
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