Please use this identifier to cite or link to this item: http://repository.vnu.edu.vn/handle/VNU_123/28992
Title: Regulation of the Biosynthesis of the Macrolide Antibiotic Spiramycin in Streptomyces ambofaciens
Authors: Nguyen, Hoang Chuong
Darbon, Emmanuelle
Karray, Fatma
Keywords: COELICOLOR A3(2);POLYKETIDE SYNTHASE;ESCHERICHIA-COLI;SACCHAROPOLYSPORA-ERYTHRAEA;TYLOSIN BIOSYNTHESIS;CONSTRUCTION
Issue Date: 2010
Publisher: AMER SOC MICROBIOLOGY, 1752 N ST NW, WASHINGTON, DC 20036-2904 USA
Citation: ISIKNOWLEDGE
Abstract: Streptomyces ambofaciens synthesizes the macrolide antibiotic spiramycin. The biosynthetic gene cluster for spiramycin has been characterized for S. ambofaciens. In addition to the regulatory gene srmR (srm22), previously identified (M. Geistlich et al., Mol. Microbiol. 6: 2019-2029, 1992), three putative regulatory genes had been identified by sequence analysis. Gene expression analysis and gene inactivation experiments showed that only one of these three genes, srm40, plays a major role in the regulation of spiramycin biosynthesis. The disruption of srm22 or srm40 eliminated spiramycin production while their overexpression increased spiramycin production. Expression analysis was performed by reverse transcription-PCR (RT-PCR) for all the genes of the cluster in the wild-type strain and in the srm22 (srmR) and srm40 deletion mutants. The results from the expression analysis, together with the ones from the complementation experiments, indicated that Srm22 is required for srm40 expression, Srm40 being a pathway-specific activator that controls most, if not all, of the spiramycin biosynthetic genes.
Description: TNS06118 ; JOURNAL OF BACTERIOLOGY Volume: 192 Issue: 21 Pages: 5813-5821
URI: http://repository.vnu.edu.vn/handle/VNU_123/28992
http://jb.asm.org/content/192/21/5813.figures-only
ISSN: 0021-9193
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