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Title: Activated platelet-rich plasma improves adipose-derived stem cell transplantation efficiency in injured articular cartilage
Authors: Pham, Phuc Van
Bui, Khanh Hong-Thien
Ngo, Quoc Dat
Keywords: Adipose tissue-derived stem cells;Articular cartilage injury;Joint failure;Mesenchymal stem cells;Osteoarthritis;Platelet-rich plasma
Issue Date: 2013
Abstract: Introduction: Adipose-derived stem cells (ADSCs) have been isolated, expanded, and applied in the treatment of many diseases. ADSCs have also been used to treat injured articular cartilage. However, there is controversy regarding the treatment efficiency. We considered that ADSC transplantation with activated platelet-rich plasma (PRP) may improve injured articular cartilage compared with that of ADSC transplantation alone. In this study, we determined the role of PRP in ADSC transplantation to improve the treatment efficiency. Methods:ADSCs were isolated and expanded from human adipose tissue. PRP was collected and activated from human peripheral blood. The effects of PRP were evaluatedin vitro and in ADSC transplantationin vivo. In vitro, the effects of PRP on ADSC proliferation, differentiation into chondrogenic cells, and inhibition of angiogenic factors were investigated at three concentrations of PRP (10%, 15% and 20%).In vivo, ADSCs pretreated with or without PRP were transplanted into murine models of injured articular cartilage. Results:PRP promoted ADSC proliferation and differentiation into chondrogenic cells that strongly expressed collagen II, Sox9 and aggrecan. Moreover, PRP inhibited expression of the angiogenic factor vascular endothelial growth factor. As a result, PRP-pretreated ADSCs improved healing of injured articular cartilage in murine models compared with that of untreated ADSCs. Conclusion:Pretreatment of ADSCs with PRP is a simple method to efficiently apply ADSCs in cartilage regeneration. This study provides an important step toward the use of autologous ADSCs in the treatment of injured articular cartilage
Description: STEM CELL RESEARCH & THERAPY Volume: 4 Article Number: 91 Published: AUG 1 2013 ; TNS07215
ISSN: 1866-6280
Appears in Collections:Bài báo của ĐHQGHN trong Web of Science

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