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  • Authors: Nguyễn, Thị Tuyền; Đinh, Thúy Hằng (2009)

  • Fe(II)-oxydizing NO3--reducing bacteria in sediment samples collected from freshwater aquifer, flooded paddy soil and estuarine area were quantified and analysed on species composition. The MPN results showed that among the three studied environments, flooded paddy soil had the highest number of these bacteria (9.3 • 103.g-1), five and two times higher than that of freshwater aquifer and estuarine sediment, respectively. Based on distinguish colony characteristics, 12 strains of Fe(II)-oxidizing, NO3-- reducing bacteria were isolated from MPN series and were set into five genetically different groups as proposed by ARDRA analyses with two restricition enzymes HaeIII and MspI, showing relatively high diversity of this bacterial group in the studied environments. Analysing species com...

  • Article


  • Authors: Nguyen, Thi Hanh; Duong, Van Hop; Nguyen, Hong Minh; Dinh, Thuy Hang (2009-04-24)

  • Via enrichment procedures carried out on a dioxin contaminated sediment sample from lotus pond at Danang airport, a microbial community assigned as DN553 with high capability of carbazol degradation was established. Analyses of community structure by using denaturing gradient gel electrophoresis (DGGE) of 16S rDNA fragments indicated that Achromobacter and Alcaligenes species dominated in this enrichment culture. In addition to carbazol, the enrichment culture was also able to utilize other PAH compounds such as naphthalene and phenanthrene as the only carbon and energy sources. In the presence of different PAH as growth substrates, the community structure changed accordingly, however the Achromobacter and Acaligenes groups still remained. Thus, the enrichment cultures DN553 could b...

  • Article


  • Authors: Nguyen, Hong Minh; Duong, Van Hop; Nguyen, Thi Hanh; Dinh, Thuy Hang (2010)

  • Bacterial community in dioxin contaminated soil and mud samples from Danang airport was enumerated and studied for their potential degradation capability against three-ring compound carbazol. The cell number was determined on nutrient rich medium and on mineral medium supplemented with carbazol. It has been showed that number of cells presented in mud samples was significantly higher than in soil samples, in the range of 1 magnitude order. About 50% of the total cell number in each sample grown on the medium with carbazol. Analyzing 12 representative strains selectively isolated on the carbazol containing medium by using the ARDRA method with two endonucleases HaeIII and MspI showed that bacterial community at the studied areas was highly diversified. However non from these isolates...