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  • Authors: Phan Thi Phuong Hoa; Satoshi Managaki; Norihide Nakada; Hideshige Takada; Akiko Shimizu; Duong Hong Anh; Pham Hung Viet; Satoru Suzuki (2011-04-13)

  • The ubiquitous application and release of antibiotics to the environment can result in bacterial antibiotic resistance, which in turn can be a serious risk to humans and other animals. Southeast Asian countries commonly apply an integrated recycling farm system called VAC (Vegetable, Aquaculture and Caged animal). In the VAC environment, antibiotics are released from animal and human origins, which would cause antibiotic-resistant bacteria (ARB). This study evaluated occurrence of ARB in the VAC environment in northern Vietnam, with quantitative analysis of antibiotic pollution. We found that sulfonamides were commonly detected at all sites. In dry season, while sulfamethazine was a major contaminant in pig farm pond (475-6662 ng/l) and less common in city canal and aquaculture site...

  • Article


  • Authors: Duong Van Hop; Yayoi Sakiyama; Chu Thi Thanh Binh; Misa Otoguro; Dinh Thuy Hang; Shinji Miyadoh; Dao Thi Luong; Katsuhiko Ando (2011-04-07)

  • Actinomycetes were isolated from 109 soil and 93 leaf-litter samples collected at five sites in Vietnam between 2005 and 2008 using the rehydration-centrifugation (RC) method, sodium dodecyl sulfate-yeast extract dilution method, dry-heating method and oil-separation method in conjunction with humic acid-vitamin agar as an isolation medium. A total of 1882 strains were identified as Vietnamese (VN)-actinomycetes including 1080 (57%) streptomycetes (the genus Streptomyces isolates) and 802 (43%) non-streptomycetes. The 16S ribosomal RNA gene sequences of the VN-actinomycetes were analyzed using BLAST searches. The results showed that these isolates belonged to 53 genera distributed among 21 families. Approximately 90% of these strains were members of three families: Streptomycetaceae...

  • Article


  • Authors: L. T. P. Hoa; D. N. Quang; N. T. H. Ha; N. H. Tri (2011)

  • Microalgae are valuable source of many unique biologically active compounds including anticancer compounds. In this study, sixteen microalgal strains were successfully isolated from mangrove in Xuanthuy National Park, Namdinh, Vietnam. Relative identification for each train was obtained based on morphological properties and 18 S rDNA sequence analysis. Culture extracts of these strains were tested against KB (human epidermal carcinoma) cell line. Ankistrodesmus gracilis VACC-010 and Amphiprora alata VACC-007 showed strongest inhibition with their IC50 values of 26.50 and 29.82 ug mL^-1, respectively. Ankistrodesmus gracilis VACC-010 was also significantly effective against HepG2 (hepatocellular carcinoma) cell line (IC50, values of 9.64 ug ml^-1), suggesting a potential source of an...