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  • Authors: Le Thi Phuong Hoa; Dang Ngoc Quang; Nguyen Hoang Tri; Nguyen Thi Hoai Ha; Pham Thi Bich Dao (2010)

  • Diatoms are predominant in marine water and considered to be the most important primary producers in sustaining marine food chains. Three diatom strains were successfully isolated from Giao Thuy mangrove water of the Red River Delta Biosphere Reserve. The morphology and the 18S rDNA sequence analysis revealed their identification as Navicula radiosa, Navicula tuscula and Melosira nummuloides. Among three media f/2, ASW and ESM, the best medium for the growth of N. radiosa and M. nummuloides is ASW whereas for N. tuscula is ESM. Fatty acid profiles indicated taxonomic similarity between three strains and other diatoms with high concentration of 16:0, 16:1n-7 and 14:0 (approximately 60 - 80% of total fatty acids). M. nummuloides contained higher content of unsaturated fatty acids (45....

  • Article


  • Authors: Le Thi Phuong Hoa; Nguyen Thi Hoai Ha; Pham Thi Bich Dao; Nguyen Ngoc Tuyen (2010)

  • A Chaetoceros strain was successfully isolated to a unialgal state from Giao Thuy mangrove and identified to be of Chaetoceros muelleri species based on morphological properties and 18S rDNA sequence analysis. ASW medium together with 25‰ of salinity was found to be best suitable for the growth of this strain and was applied to its biomass production. The fatty acid profile of C. muelleri strain was typical of most diatoms with the exception of low level of 16:0 and 14:0 acids. This strain possessed high concentration of polyunsaturated fatty acids (PUFAs), 36.64% total fatty acids, which is much higher than that of C. calcitrans, C. gracilis and C. muelleri in previous reports. Among these acids, eicosapentaenoic acid (EPA) and arachidonic acid (AA) had significant proportion (24.7...

  • Article


  • Authors: Le Thi Phuong Hoa; Nguyen Thi Hoai Ha; Dang Ngoc Quang; Nguyen Hoang Tri (2010)

  • Microalgae play an important part in primary production in mangrove ecosystem. Many of them possess high content of polyunsaturated fatty acids (PUFAs) as well as other compounds of potential interest to the food, cosmetic and pharmaceutical industries. Three diatom strains were successfully purified from Giaothuy mangrove forest and taxonomically identified as Amphiprora alata, Gyrosigma limosum and Melosira nummuloides according to morphological properties and 18S rDNA sequence analysis. Predominant fatty acids in three strains were 16:0, 16:1n-7 and 14:0, as similar as other diatoms. They contained high concentration of unsaturated acids (approximately 45-52% of total fatty acids), among which PUFAs accounted for 30% -50%. Eicosapentaenoic acid (EPA), which is a precursor in pros...