Development of a cancer cell manipulating and sensing microfluidic platform

Abstract

The thesis has studied and proposed two microfluidic structures including fluidic chamber structure and microfluidic channel structure. The structure of the fluidic chamber is capable of manipulating, enriching, detecting and counting cancer cells. The microfluidic channel structure allows the count of lung cancer cells which pass through the microfluidic channel. Proposed structures are successfully designed and fabricated based on standard microfabrication technology. The operation of the proposed structure model has been successfully simulated and prototypes have been designed, fabricated and evaluated. The thesis has also successfully designed and fabricated electronic modules for controlling manipulating, enriching cancer cells in fluidic chambers and modules for processing acquired electronic signal in order to detect and count biological cells in fluidic chambers and in microfluidic channels. This study successfully integrated electronic circuit modules with two proposed structures to manipulate, enrich, detect and enumerate the biological cells. The results obtained from the experiments are in align with the simulation results.